Journal: Oncology Letters

Article Title: Effects of Yangzheng Sanjie Decoction-containing serum mediated by microRNA-7 on cell proliferation and apoptosis in gastric cancer

doi: 10.3892/ol.2018.7757

Figure Lengend Snippet: Expression of miR-7 and EGFR. (A) Hematoxylin staining indicating the expression of miR-7 by in situ hybridization (magnification, ×400). (B) Expression of miR-7 for reverse transcription-quantitative polymerase chain reaction. U6 was used as the internal control. (C) Western blot analysis of EGFR. (D) Representative image of EGFR for western blot analysis. β-actin was used as the internal control. *P<0.05. miR-7, microRNA-7; EGFR, epidermal growth factor receptor; CM, Chinese medicine; Con, control; GC, gastric cancer.

Article Snippet: The membranes were blocked with 5% milk in Tris-buffered saline and 0.2% Tween at room temperature for 1 h, prior to being incubated overnight at 4°C with specific primary antibodies against the following: β-actin (dilution, 1:250; cat. no. BM0627; Boster Biological Technology, Wuhan, China) and EGFR (dilution, 1:1,000; cat. no. 4267S; CST Biological Reagents Co., Ltd., Shanghai, China).

Techniques: Expressing, Staining, In Situ Hybridization, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Western Blot